Next Generation Sequencing in A375 and SK-MEL-28 cell lines

RNA was extracted from 12 samples from two melanoma cell lines using MagZol reagent with standard protocol. Overall design: RNA was extracted from 12 samples from two melanoma cell lines using MagZol reagent with standard protocol. We conducted drug sensitivity assays on selected drugs in two different melanoma cell lines (A375 and SK-MEL-28) under a rapamycin-induced autophagy condition versus a control condition.

使用MagZol试剂并遵循标准实验流程，从两种黑色素瘤细胞系的12个样本中提取了RNA。实验整体设计：使用MagZol试剂并遵循标准实验流程，从两种黑色素瘤细胞系的12个样本中提取了RNA。我们针对两种不同的黑色素瘤细胞系（A375与SK-MEL-28）中的选定药物开展了药物敏感性检测，实验设置了雷帕霉素诱导自噬条件组与对照组两种处理方式。