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Anaplasma is an intracellular alphaproteobacteria that infects diverse blood cell types in animal hosts including small ruminants. Epidemiological and risk factors information on zoonotic anaplasmosis with respect to anaplasmosis in sheep and goats are scarce. Therefore, the objective of the current study was to estimate the prevalence, risk factors of anaplasmosis and phylogenetic investigation of A. capra in sheep and goats from Faisalabad district, Pakistan. Briefly, 384 blood samples were randomly collected from sheep and goats of Faisalabad district, Pakistan, during January to May 2022. The samples were processed for the detection of Anaplasma targeting 16S rRNA gene using PCR. The data regarding disease determinants were collected using a predesigned questionnaire. Out of 384 samples, 131 samples were found positive for Anaplasma spp. with a prevalence rate of 34.11%. The results indicated a significantly higher prevalence of anaplasmosis in goats (41.88%) compared to sheep (22.00%). In addition, the chi square indicated that housing type, tick infestation, gender, tick control practices, age, mix farming, and hygiene were significantly associated with the occurrence of disease. The analysis of multivariate logistic regression expressed gender as the significant risk factor (p = 0.0001, OR = 1.757, CI = 1.305–2.366). The acquired sequences revealed four novel isolates of A. capra (Genbank accession numbers ON834323, ON838209, ON838210, and ON838211). The phylogenetic analysis of the 16S rRNA gene of A. capra revealed three distinct clusters with 99–100% homology with other isolates from different countries. Our isolates showed higher similarity with isolates from China (KM206273, KP314237, MT799937), Pakistan (ON238129, ON238130, ON238131), Angola (MT898988), India (MZ558066), Iran (MW692362), and Turkey (MT632469) isolated from human, sheep, ticks, goats, cattle, Gaddi goat, Persian Onager (Equus hemionus onager), and Turkish goats, respectively. In conclusion, A. capra is endemic in Punjab, Pakistan, there is a need to conduct large scale surveillance studies to assess the status of this pathogen at human-animal interface as well as to develop effective preventive and control strategies to reduce the economic losses associated with anaplasmosis in small ruminants.无形体属（Anaplasma）是一类胞内寄生的α-变形菌，可感染包括小型反刍动物在内的多种动物宿主的血细胞。目前针对绵羊和山羊无形体病的人兽共患无形体病流行病学及危险因素相关研究较为匮乏。因此，本研究旨在针对巴基斯坦费萨拉巴德县的绵羊与山羊，开展无形体病流行率调查、危险因素分析及卡氏无形体（A. capra）的系统发育研究。简要来说，本研究于2022年1月至5月期间，从巴基斯坦费萨拉巴德县的绵羊与山羊群体中随机采集了384份血液样本。采用靶向16S核糖体RNA基因的聚合酶链式反应（PCR）对样本进行无形体属检测。通过预先设计的调查问卷收集疾病相关危险因素数据。在384份样本中，共131份样本呈无形体属阳性，整体流行率为34.11%。结果显示，山羊的无形体病流行率（41.88%）显著高于绵羊（22.00%）。此外，卡方检验结果表明，饲养方式、蜱虫感染情况、宿主性别、蜱虫防控措施、年龄、混合养殖及卫生状况均与该病的发生存在显著关联。多因素logistic回归分析显示，性别为该病的显著危险因素（p=0.0001，比值比OR=1.757，置信区间CI=1.305–2.366）。本研究获得的序列共鉴定出4株卡氏无形体（A. capra）新分离株，其GenBank登录号分别为ON834323、ON838209、ON838210及ON838211。对卡氏无形体16S rRNA基因的系统发育分析显示，其可分为3个独立进化簇，与来自不同国家的其他分离株的同源性达99%–100%。本研究的分离株与以下来源的分离株同源性较高：中国（KM206273、KP314237、MT799937，分别分离自人类、绵羊、蜱虫）、巴基斯坦（ON238129、ON238130、ON238131，分离自山羊）、安哥拉（MT898988，分离自牛）、印度（MZ558066，分离自加迪山羊）、伊朗（MW692362，分离自波斯野驴（Equus hemionus onager））及土耳其（MT632469，分离自土耳其山羊）。综上，卡氏无形体（A. capra）在巴基斯坦旁遮普省呈地方性流行，后续需开展大规模监测研究以评估该病原体在人畜共患界面的流行状况，并制定有效的防控策略以降低小型反刍动物无形体病造成的经济损失。