Mutant-CCAAT/enhancer-binding protein alpha target genes in myeloid progenitor cells by chromatin IP. Mus musculus

CCAAT/enhancer-binding protein alpha (C/EBPα) is a lineage-specific transcription factor that directs development of granulocytes. To obtain insights into the genome-wide transcriptome of mutant C/EBPα, we have performed chromatin immunoprecipitation on DNA promoter microarrays (ChIP-chip) in the murine 32D myeloid progenitor cell line. These cells expressed an estradiol-inducible form of mutant C/EBPα. Overall design: In the murine 32D myeloid progenitor cell line, expressing an estradiol-inducible form of C-terminal mutant of C/EBPα. (n=2, replicates). We have performed chromatin immunoprecipitation on DNA promoter microarrays (ChIP-chip) after 4 hours of estradiol incubation.

CCAAT/增强子结合蛋白α（CCAAT/enhancer-binding protein alpha，C/EBPα）是一类谱系特异性转录因子，可调控粒细胞的分化发育。为深入解析突变型C/EBPα的全基因组转录组特征，我们以表达雌二醇诱导型突变C/EBPα的小鼠32D髓系祖细胞系为模型，开展了染色质免疫沉淀联合DNA启动子微阵列（ChIP-chip）实验。整体实验设计：本实验采用表达雌二醇诱导型C端突变C/EBPα的小鼠32D髓系祖细胞系，设置2次生物学重复（n=2）；在雌二醇孵育4小时后，进行染色质免疫沉淀联合DNA启动子微阵列（ChIP-chip）检测。