Nick Dolman, Rob Batchelor, Penny Melquist, Mike O'Grady, George Hanson (2011) CIL:7753, Homo sapiens. CIL. Dataset

Mitosis is visualized here in a U2OS human osteosarcoma cell transduced with 'Cellular Lights' Histone 2B-RFP to mark the nucleus red and 'Cellular Lights' MAP4-GFP (MAP4 is a microtubule associated protein) and imaged the next day. In this video, microtubules appear green and histone proteins in the nucleus appear red. Cells were imaged on a Deltavision core microscope with a 40X objective images were collected every 5 minutes for 16 hours. Fluorescence was detected using the appropriate filters (FITC/TRITC). Scale bar = 20 microns.

本数据集展示了通过基因转导技术将人骨肉瘤细胞系U2OS导入'Cellular Lights'组蛋白2B-RFP（用以标记细胞核呈现红色）和'Cellular Lights' MAP4-GFP（MAP4为微管相关蛋白）的细胞有丝分裂过程。在随后的视频中，微管以绿色呈现，而细胞核中的组蛋白蛋白以红色显现。细胞图像是在配备40倍物镜的Deltavision核心显微镜下拍摄的，图像每5分钟收集一次，持续16小时。荧光检测是通过合适的滤光片（FITC/TRITC）完成的。比例尺为20微米。