Endogenous CK levels (pmol g⁻¹ fresh weight) in leaves and roots of T1 <i>PHT::ZmCKX1</i> barley plants.

The CKs analyzed included: free bases of trans-zeatin (tZ), cis-zeatin (cZ), dihydrozeatin (DHZ) and N6-isopentenyladenine (iP); their N9-ribosides (tZR, cZR, DHZR, iPR); O-glucosides (tZOG, cZOG, DHZOG); O-glucoside-N9-ribosides (tZROG, cZROG, DHZROG); N9-glucosides (tZ9G, cZ9G, DHZ9G, iP9G); and N9-riboside-5′-monophosphates (tZR5′MP, cZR5′MP, DHZ5′MP, iPR5′MP); sums of free bases and N9-ribosides are considered to comprise the pool of active CKs; mean values with standard deviations obtained from three biological replicates are presented; asterisks indicate significant differences between control and transformed tissue according to Student's unpaired t-tests at P≤0.05; UDL – under detection limit; WT – plants germinated from wild-type grains.本次分析的细胞分裂素（cytokinins, CKs）包含以下形式：反式玉米素（trans-zeatin, tZ）、顺式玉米素（cis-zeatin, cZ）、二氢玉米素（dihydrozeatin, DHZ）与N6-异戊烯基腺嘌呤（N6-isopentenyladenine, iP）的游离碱基形式；其对应的N9-核糖苷（tZR、cZR、DHZR、iPR）、O-葡萄糖苷（tZOG、cZOG、DHZOG）、O-葡萄糖苷-N9-核糖苷（tZROG、cZROG、DHZROG）、N9-葡萄糖苷（tZ9G、cZ9G、DHZ9G、iP9G）以及N9-核糖苷-5′-单磷酸酯（tZR5′MP、cZR5′MP、DHZ5′MP、iPR5′MP）。其中，游离碱基与N9-核糖苷的总和被视为活性细胞分裂素库的组成部分。本研究呈现了3次生物学重复实验得到的平均值与标准差。星号代表依据未配对Student t检验，对照组与转化组组织间存在P≤0.05水平的显著差异。UDL（under detection limit）即低于检测限；WT（wild-type）指由野生型种子萌发得到的植株。