Mechanistic study on direct and indirect thyroid toxicity in male Wistar rats - Serum Metabolomics

The present oral toxicity study in male Wistar rats is focusing on direct and indirect thyroid<br> toxicity. Biomaterials from this study were used to generate transcriptomics, proteomics,<br> and metabolomics data sets. Featuring a time- and concentration-resolved design<br> including recovery after treatment, this study allows investigating adaptive versus adverse<br> effects in several dimensions.<br> Two well-described test chemicals, i.e., Phenytoin and Propylthiouracil, were administered<br> at each two dose levels to male Wistar rats for 2 (Subset A) and 4 weeks via the diet<br> (Subset B). In addition, a recovery period of 2 weeks without test substance application was<br> included, in order to monitor reversibility of the induced effects (Subset C).<br> During the course of the study, blood samples were taken for metabolome analyses.

本研究针对雄性Wistar大鼠开展经口毒性试验，聚焦甲状腺的直接与间接毒性效应。本研究获取的生物样本被用于生成转录组学（transcriptomics）、蛋白质组学（proteomics）及代谢组学（metabolomics）数据集。该研究采用时间与浓度分辨率设计，并设置给药后恢复期，可从多维度探究受试物的适应性效应与不良效应。
本研究选用两种经过充分表征的受试化学品：苯妥英（Phenytoin）与丙基硫氧嘧啶（Propylthiouracil），以两种剂量水平通过饮食途径给雄性Wistar大鼠给药，分别给药2周（子集A）与4周（子集B）。
此外，本研究设置为期2周的无受试物给药恢复期（子集C），以监测受试物诱导效应的可逆性。
试验期间采集血液样本，用于代谢组分析。