BIO407 Group2 Image processing of live cells, treated with DFX

## Study:This data was acquired for the paper:Gottwald EM, Schuh CD, Drücker P, Haenni D, Pearson A, Ghazi S, Bugarski M, Polesel M, Duss M, Landau EM, Kaech A, Ziegler U, Lundby AKM, Lundby C, Dittrich PS, Hall AM. The iron chelator Deferasirox causes severe mitochondrial swelling without depolarization due to a specific effect on inner membrane permeability. Sci Rep. 2020 Jan 31;10(1):1577. doi: 10.1038/s41598-020-58386-9. PMID: 32005861; PMCID: PMC6994599. ## Study Component:Part of Fig.1c&dConfocal timelapse of Deferasirox treated cells, showing mitochondria swelling over time and polarization loss. ## Biosample:Opossum kidney (OK) cells (kind gift from the group of Prof O. Devuyst (Physiology, University of Zurich)) ## Specimen:DFX treatment: 200uMDyes:-Mitochondria-GFP BacMam 2.0-TMRM (mitochondrial membrane potential dependent dye) ## Image Acquisition:Images were acquired using a Leica SP8 inverse STED 3x.Acquired channels:Channel 1: Mitochondria-GFP, 488nm excitation, 493nm-548nm emissionChannel 2: TMRM, 553nm excitation, 564nm-650nm emissionObjective: HC PL APO CS2 100x/1.40 OIL ## Image Data:151106_slide1_bacmam20_PPC_7473_Esther_DH_Progression.lif Original project number:7473Study: 本数据集源自以下研究论文：Gottwald EM、Schuh CD、Drücker P、Haenni D、Pearson A、Ghazi S、Bugarski M、Polesel M、Duss M、Landau EM、Kaech A、Ziegler U、Lundby AKM、Lundby C、Dittrich PS、Hall AM。论文题为《铁螯合剂地拉罗司（Deferasirox，DFX）通过对内膜通透性的特异性作用引发严重线粒体肿胀而不发生膜电位去极化》，发表于《科学报告》（Sci Rep），2020年1月31日；10(1):1577。DOI: 10.1038/s41598-020-58386-9，PMID: 32005861；PMCID: PMC6994599。 Study Component: 研究组分：包含图1c与1d的部分内容，即地拉罗司处理细胞的共聚焦延时成像（Confocal timelapse），可观测到随时间推移发生的线粒体肿胀与膜电位丧失。 Biosample: 生物样本：负鼠肾（Opossum Kidney, OK）细胞，由苏黎世大学生理学系O. Devuyst教授课题组惠赠。 Specimen: 标本处理：地拉罗司（Deferasirox，DFX）处理浓度为200μM；所用染料：① 线粒体-GFP（Mitochondria-GFP）BacMam 2.0；② TMRM（线粒体膜电位依赖性染料）。 Image Acquisition: 图像采集：采用徕卡（Leica）SP8倒置STED 3x显微镜完成图像采集。采集通道设置如下：通道1：线粒体-GFP，激发波长488nm，发射波长范围493nm~548nm；通道2：TMRM，激发波长553nm，发射波长范围564nm~650nm；所用物镜：HC PL APO CS2 100x/1.40 OIL。 Image Data: 图像数据文件：151106_slide1_bacmam20_PPC_7473_Esther_DH_Progression.lif；原始项目编号：7473。