Sperm and seminal fluids proteomes in selected passerine bird species

This project contains sperm and seminal fluids proteomes from selected species of passerine birds: Taeniopygia guttata, Hirundo rustica, Chloris chloris, Acrocephalus palustris, Cinclus cinclus, Phylloscopus collybita. We employed label-free proteomics to generate proteomes in the zebra finch, a songbird species with annotated genome and five wild-living songbirds representing five families within the Passerida clade, all with poorly known genomes. The results show that protein mapping of the new passerine proteomes to zebra finch genome was successful, thus, yielding highly similar protein identifications and a sufficient number of unique peptides in all the studied proteomes.

本数据集包含雀形目选定鸟类的精子与精浆蛋白质组，涉及物种为：斑胸草雀（Taeniopygia guttata）、家燕（Hirundo rustica）、绿金翅雀（Chloris chloris）、芦苇莺（Acrocephalus palustris）、河乌（Cinclus cinclus）以及褐柳莺（Phylloscopus collybita）。本研究采用无标记蛋白质组学（label-free proteomics）技术，为已完成基因组注释的鸣禽物种斑胸草雀，以及隶属于Passerida演化支5个不同科的5种野生鸣禽构建了蛋白质组；上述5种野生鸣禽的基因组信息目前均较为匮乏。研究结果表明，将本次新获得的雀形目蛋白质组与斑胸草雀基因组进行蛋白映射的实验取得成功，最终在所有受试蛋白质组中均得到了高度相似的蛋白鉴定结果，且获得了足够数量的独特肽段（unique peptides）。