A genome-wide CRISPR screen for genes regulating ferroptosis sensitivity. A genome-wide CRISPR screen for genes regulating ferroptosis sensitivity

Genome-wide CRISPR screens were performed in Huh-7 hepatoma cell line to identify genes regulating ferroptosis sensitivity. Huh-7 cells were transduced with Human GeCKO library and selected by RSL-3. Overall design: Genome-wide CRISPR screen (GeCKOv2 library) in Huh-7 cells teather reated with RSL-3 or linoleic acid and RSL-3

本研究在Huh-7肝癌细胞系（Huh-7 hepatoma cell line）中开展全基因组CRISPR筛选（Genome-wide CRISPR screens），以鉴定调控铁死亡敏感性（ferroptosis sensitivity）的基因。具体操作：将人类GeCKO文库（Human GeCKO library）转导至Huh-7细胞，随后通过RSL-3进行筛选。实验整体设计：在经RSL-3或亚油酸（linoleic acid）联合RSL-3处理的Huh-7细胞中，采用GeCKOv2文库（GeCKOv2 library）开展全基因组CRISPR筛选。