Genioglossus EMG metrics from male Sprague-Dawley rats during electrical stimulation after C2 hemisection and C1 transection injury

STUDY PURPOSE: The purpose of this study was to understand how electrical stimulation at the spinal level C4, targeting the phrenic motor nucleus, affects hypoglossal motor output under varying levels of injury. DATA COLLECTED: In six 3-6 month old urethane anesthetized male Sprague Dawley rats, EMG recording electrodes were placed on the base of the tongue, and stimulating electrodes on the dorsal surface of C4. Rats first underwent a right-sided C2 hemisection before undergoing short bouts of varying amplitude electrical stimulation timed during the breath, between breaths, and throughout the respiratory cycle as determined by hypoglossal EMG. Shortly after completion of stimulation under C2HS conditions, rats then underwent a complete C1 transection, and the stimulus bouts were repeated. We evaluated changes in hypoglossal EMG amplitude during and after stimulation bouts, as well as the degree of EMG entrainment to stimulation pulses via stimulus triggered averages of the raw EMG signals. DATA USAGE NOTES:

研究目的：本研究旨在阐明脊髓C4节段靶向膈运动核的电刺激，在不同损伤程度下对舌下运动输出的影响。
实验采集数据：选取6只3~6月龄的乌拉坦麻醉雄性斯普拉格-道利（Sprague Dawley）大鼠，将肌电图（electromyography，EMG）记录电极置于舌根部，刺激电极置于C4节段背侧表面。大鼠首先接受右侧C2半切术，随后在呼吸时相、呼吸间歇期及全呼吸周期（由舌下EMG判定）施加不同振幅的短时电刺激。在C2半切条件下完成刺激实验后，随即对大鼠施行完全性C1横断术，并重复上述刺激实验流程。本研究评估了刺激过程中及刺激结束后舌下EMG振幅的变化，同时通过对原始EMG信号进行刺激触发平均分析，探究了EMG与刺激脉冲的同步耦合程度。
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