Essential functions of the Glucocorticoid Receptor require direct DNA binding [RNA-seq]

We performed RNA sequencing in Mouse Embryonic Fibroblasts (MEFs) of GR wild-type and GR Zn. Cells were treated with: vehicle (EtOH), Dexamethasone (Dex), Lipopolysaccharide (LPS) or Dex+LPS. We show that GR Zn does not regulate any GR target gene mRNA profile of GR wild-type and GR Zn MEFs treated with vehicle, Dex, LPS or DexLPS

我们对糖皮质激素受体（Glucocorticoid Receptor，GR）野生型及GR Zn型小鼠胚胎成纤维细胞（Mouse Embryonic Fibroblasts，MEFs）进行了RNA测序（RNA sequencing）实验。将该类细胞分别以赋形剂（乙醇，EtOH）、地塞米松（Dexamethasone，Dex）、脂多糖（Lipopolysaccharide，LPS）以及地塞米松联合脂多糖（Dex+LPS）进行处理。本研究结果表明，在分别经赋形剂、地塞米松、脂多糖或地塞米松联合脂多糖处理的GR野生型与GR Zn型MEFs中，GR Zn均未调控任何GR靶基因的mRNA表达谱。