The bacteriology of pleural infection using next generation sequencing: The Oxford Pleural Infection Metagenomics Studies (TORPIDS). The microbiology of pleural infection

Pleural infection is a complicated, severe and frequent disease worldwide. The knowledge of pleural infection microbiology remains incomplete. We designed a translational metagenomics study to investigate the bacteriology of pleural infection. Pleural fluid biospecimens from the Prospective validation of the RAPID clinical risk prediction score in adult patients with pleural infection: the PILOT study were subjected to bacterial DNA extraction and 16S rRNA next generation sequencing. Pleural fluid samples from patient without pleural infection were used as negative control.

胸膜感染是一种全球范围内高发、病情复杂且严重的疾病。目前学界对胸膜感染的微生物学认知仍存在不足。本研究设计了一项转化宏基因组学研究，以探究胸膜感染的细菌学特征。本研究纳入了来自《成人胸膜感染患者RAPID临床风险预测评分的前瞻性验证：PILOT研究》的胸腔液生物标本，对其进行细菌DNA提取及16S rRNA高通量测序。将未罹患胸膜感染患者的胸腔液样本设为阴性对照。