EWS siRNA knock-down in HeLa cell line. Homo sapiens

HeLa cell line was trasfected by a EWS siRNA oligo causing knock-down of EWS or a siRNA scrambled oligo Overall design: Three biological replicates were labeled in direct and dye-swap microarray experiments and hybridized onto an Agilent custom splicing-sensitive microarray platform.

本数据集涉及的海拉细胞系（HeLa）分别经两种转染处理：转染EWS小干扰RNA（siRNA）寡核苷酸以实现EWS基因敲低，或转染乱序siRNA寡核苷酸作为阴性对照。
实验整体设计：共设置3个生物学重复，采用直接标记与染料互换的微阵列实验方案，并将标记后的样本杂交至安捷伦（Agilent）定制剪接敏感型微阵列平台。