Short chain fatty acid-mediated epigenetic modulation of inflammatory T cells in vitro

The present study examined the modulation of chromatin accessibility via ATACseq of CD4 T cells expanded in Th17 inducing conditions in the presence of the short chain fatty acid butyrate. Four groups were tested, Th17 conditions alone, and Th17 conditions with one of the following: 0.5 mM butyrate, 1 mM butyrate, or liposomal encapsulated butyrate (BLIPs). The goal was to enhance butyrate delivery via liposomal encapsulation, and demonstrate that BLIP treatment resulted in the same chromatin modulation as unencapsulated butyrate in CD4 T cells.

本研究采用ATAC测序（ATAC-seq）技术，分析了在Th17诱导条件下、添加短链脂肪酸丁酸的环境中扩增得到的CD4 T细胞的染色质可及性调控变化。实验共设置四组测试样本：仅Th17诱导条件组，以及分别添加0.5 mM丁酸、1 mM丁酸或脂质体包封丁酸（BLIPs）的Th17诱导条件组。本研究旨在通过脂质体包封技术提升丁酸的细胞递送效率，并验证BLIPs处理可在CD4 T细胞中实现与未包封丁酸一致的染色质调控效果。