Supporting data for "Characterization of the Cancer Stemness Effect of gene MAEL on HCC"

Hepatocellular carcinoma is the worldwide health problem. According to analysis the RNA sequencing of three paired HCC and adjacent non-tumor samples, we found several oncogenes played critical roles in development and progression on HCC. Our previous study has demonstrated that gene MAEL was up-regulated in tumor samples compared with matched no-tumor smples. High expression of MAEL associated with HCC metastasis and poor overall survival rate. Functional study showed overexpression of MAEL increased cell growth, mobility, which medicated by AKT pathways activity and inducing EMT. Moreover, MAEL transfected cell upregulated stemness related gene and CSC surface markers in mRNA level as well as enriched CD133 percentage. The dataset includes the Flow cytometry results, IF staining, In vitro functional studies, tumor formation in nude mice, Sorafenib treatment ELISA and RNA-Seq as the data information txt of each file folder/

肝细胞癌（Hepatocellular carcinoma, HCC）是全球性的公共卫生挑战。本研究通过对3对HCC组织及其配对的癌旁正常组织开展RNA测序分析，发现多个癌基因在HCC的发生与进展过程中发挥关键调控作用。我们前期研究已证实，相较于配对的正常组织，MAEL基因在肿瘤组织中呈高表达。MAEL基因的高表达与HCC的转移及患者不良总体生存率显著相关。功能实验结果显示，MAEL基因过表达可促进细胞增殖与迁移，该过程由AKT通路活化介导并诱导上皮间质转化（epithelial-mesenchymal transition, EMT）。此外，转染MAEL基因的细胞在mRNA水平上可上调干细胞相关基因及癌症干细胞（Cancer Stem Cell, CSC）表面标志物的表达，同时富集CD133阳性细胞比例。本数据集包含各文件夹下的以下各类数据信息文本：流式细胞术（Flow Cytometry）检测结果、免疫荧光（IF）染色结果、体外功能实验数据、裸鼠成瘤实验数据、索拉非尼（Sorafenib）处理相关ELISA检测数据以及RNA测序（RNA-Seq）数据。