Stony coral tissue loss disease intervention with amoxicillin leads to a reversal of disease-modulated gene expression pathways

An in situ experiment was conducted in Southeast Florida to assess transcriptomic responses prior to and two weeks following application of CoreRx Base 2B with amoxicillin on SCTLD-affected Montastraea cavernosa. A complementary ex situ disease challenge experiment was conducted to expose fragments of M. cavernosa and Orbicella faveolata to SCTLD-affected donor corals in order to compare transcriptomic profiles among clonal individuals from disease-unexposed, exposed/displaying disease signs, and exposed/not displaying disease signs treatment groups. This repository contains raw sequence files associated with both experiments, prepared using the Tag-Seq pipeline.

本研究在佛罗里达东南部开展了一项原位实验，旨在评估感染石珊瑚组织损失病（Stony Coral Tissue Loss Disease，SCTLD）的洞穴大星珊瑚（Montastraea cavernosa）在施用含阿莫西林的CoreRx Base 2B药剂前，以及施药后两周的转录组响应。为对比未暴露于病害、暴露且表现病害症状、暴露但未表现病害症状三个处理组中克隆个体的转录组谱，本研究同步开展了补充离体病害挑战实验：将洞穴大星珊瑚（Montastraea cavernosa）与瘤扁脑珊瑚（Orbicella faveolata）的组织片段暴露于感染SCTLD的供体珊瑚。本数据集存储库包含上述两项实验相关的原始序列文件，所有文件均通过标签测序（Tag-Seq）流程制备。