Transriptomics response to luteolin exposure on the beta rhizobium Cupriavidus necator UYPR2.512. Cupriavidus necator strain:UYPR2.512

Cupriavidus necator UYPR2.512 is a rhizobium that belongs to the beta subclass of proteobacteria, known for its ability to establish successful symbiosis with legumes of the Mimosoid clade. In most of the studied rhizobium-legumes symbioses, the initial steps of the interaction involve the reciprocal recognition by chemical signals at the rhizosphere level. The flavone luteolin is one of the molecules shown to be involved in the initial recognition steps between legumes and rhizobia. However, there is scarce information on the effect of luteolin in beta rhizobia and no data about its effect at genome-wide level. In this work we used RNA-Seq approach to analyze the transcriptional response of this strain to luteolin, as a proxy to study the first steps in the interaction between beta rhizobia and host legumes.

耐铜嗜菌（Cupriavidus necator）菌株UYPR2.512是一种根瘤菌（rhizobium），隶属于变形菌门（Proteobacteria）β亚纲，其可与含羞草类群（Mimosoid clade）的豆科植物建立稳定共生关系，这一特性已为学界所熟知。在多数已被研究的根瘤菌-豆科植物共生体系中，互作的初始步骤依赖于根际层面的化学信号介导的双向识别。黄酮类化合物木犀草素（luteolin）已被证实是参与豆科植物与根瘤菌初始识别过程的信号分子之一。然而，目前关于木犀草素对β亚纲根瘤菌的影响相关研究数据较为匮乏，且尚无全基因组层面的相关研究资料。本研究采用RNA测序（RNA-Seq）技术，分析该菌株对木犀草素的转录响应，以此作为探究β亚纲根瘤菌与宿主豆科植物互作初始步骤的替代研究手段。