TCF7L1 knockdown in pancreatic cancer

RNAi mediated depletion of TCF7L1 increases activity of a Wnt-based reporter and imparts more aggressive tumor phenotypes in vitro in pancreatic cancer cell lines that express TCF7L1. We sought to determine what changes in transcription ocurred after RNAi mediated knockdown of TCF7L1 in these cells by RNA-sequencing Overall design: 12 samples, 4 different conditions with 3 replicates each

RNA干扰（RNAi）介导的TCF7L1（TCF7L1）敲低可增强Wnt通路报告基因的活性，并在表达TCF7L1的胰腺癌细胞系的体外实验中赋予细胞更具侵袭性的肿瘤表型。本研究旨在通过RNA测序（RNA-sequencing）技术，探究上述细胞在经RNA干扰介导的TCF7L1敲低后转录组发生的变化。实验整体设计：共12个样本，分为4种不同实验条件，每种条件设置3次生物学重复。