Fibroblast and pre-osteoblast cell adhesive behavior on titanium alloy coated with diamond film

It is well known that titanium alloys have mechanical strengths comparable to steels, as well as high corrosion resistance. Also, they have the advantage of promoting osseointegration, when used in medical and dental implants. This work aims to describe the adhesion properties of fibroblast and osteoblast cells on the surface of titanium aluminum vanadium alloy (Ti6Al4V). Three different conditions of the surface were investigated: smooth, rough and covered with diamond film. Conventional material characterizations were performed to the film which consisted in: Morphological visualization by scanning electron microscopy, confocal profilometry, X-ray diffraction pattern, Raman backscattering spectroscopy and atomic force microscopy. Biocompatibility tests of Ti6Al4V were performed using primary human fibroblasts and mouse pre-osteoblasts cell line MC3T3-E1. Overall, diamond films deposited on Ti6Al4V showed interesting results of uniformity and protection against cracks on to the surface, reasonable biocompatibility features if compared to uncovered ones, indicating that this film is an alternative for using in health care applications.

众所周知，钛合金具备与钢材相当的力学强度，同时拥有优异的耐蚀性能。此外，当其应用于医用及牙科植入物时，还可促进骨整合(osseointegration)，这是其显著优势。本研究旨在探究钛铝合金(Ti6Al4V)表面上成纤维细胞(fibroblast)与成骨细胞(osteoblast)的黏附特性。本次研究共考察了三种表面状态：光滑表面、粗糙表面以及覆有金刚石薄膜的表面。针对该金刚石薄膜，采用常规材料表征手段进行分析，具体包括：扫描电子显微镜(SEM)形貌表征、共聚焦轮廓仪检测、X射线衍射(XRD)图谱分析、拉曼背散射光谱测试以及原子力显微镜(AFM)表征。针对Ti6Al4V的生物相容性测试，采用原代人成纤维细胞与小鼠前成骨细胞系MC3T3-E1开展实验。综合来看，沉积于Ti6Al4V表面的金刚石薄膜展现出良好的均匀性与表面抗开裂性能，相较于未覆膜的试样，其生物相容性更为优异，表明该薄膜可作为医疗健康领域应用的备选方案。