Novel XBP1s-independent function of IRE1 RNase in HIF-1a-mediated glycolysis upregulation in human macrophages

We investigated the immunometabolic reprogramming in SFA-treated human macrophages. As expected, RNA sequencing highlighted a pro-inflammatory profile but also metabolic signatures including glycolysis and hypoxia as well as a strong unfolded protein response. Overall design: We then performed comparative gene expression profiling analysis of RNA-seq data for human monocyte-derived macrophages(MDMs) treated with BSA (Bovin serum albumin, vehicle) and MDMs treated with a saturated fatty acid (C18:0, 100 µM) for 16h. Three replicates are included.

我们探究了经饱和脂肪酸（Saturated Fatty Acid, SFA）处理的人巨噬细胞的免疫代谢重编程过程。正如预期所示，RNA测序（RNA sequencing）不仅揭示了促炎表型，还发现了包括糖酵解、缺氧以及显著的未折叠蛋白反应在内的代谢特征。
实验整体设计：我们随后对两组人类单核细胞源性巨噬细胞（monocyte-derived macrophages, MDMs）的RNA测序（RNA-seq）数据开展了比较基因表达谱分析：一组经牛血清白蛋白（Bovine Serum Albumin, BSA，溶剂对照）处理，另一组经饱和脂肪酸（C18:0，100 μM）处理16小时。本实验设置三个生物学重复。