Pearson’s correlation between the number of mapped reads obtained from libraries 3A and 3B using different primer pairs.

All = all species and all primer pairs; All mammals = all primer pairs including only mammalian species; All avian species = all primer pairs including only avian species; when primer pairs are indicated, all species were used in the correlation; the different primer pairs were also considered separately for mammalian and avian species as indicated. Correlation between the results of the two libraries obtained with primer pair 16S_Ki for the avian species is biased due to the lack of sequenced reads in birds.
Pearson’s correlation between the number of mapped reads obtained from libraries 3A and 3B using different primer pairs.

"All" 指代所有物种与所有引物对（primer pair）；"All mammals" 指代仅包含哺乳类物种的所有引物对；"All avian species" 指代仅包含鸟类物种的所有引物对。当明确指定引物对时，相关分析将采用全部物种；同时可如前文所述，分别针对哺乳类与鸟类物种，对不同引物对单独进行分析。针对鸟类物种、使用引物对16S_Ki制备的两个测序文库的结果之间的相关性存在偏倚，原因是鸟类样本的测序读段（sequenced reads）不足。不同引物对构建的文库3A与3B的比对读段（mapped reads）数量之间的皮尔逊相关系数（Pearson’s correlation）。