PCR conditions for Sanger sequencing and ASLNAqPCR.

Ex, exon; aFor KRAS amplification a touch-down PCR was performed; bPCR for wild type KRAS and all 7 codon 12 and 13 mutations; cPCR for wild type BRAF and BRAF V600E; *Plate reading step.Ex：外显子（exon）；a. 针对KRAS扩增，采用降落PCR（touch-down PCR）完成实验；b. 用于检测野生型KRAS以及全部7种12号和13号密码子突变的PCR检测体系；c. 用于检测野生型BRAF及BRAF V600E突变的PCR检测体系；* 板读步骤。