Binding affinities of mutant dengue capping enzyme proteins for different diphosphorylated RNA species and GTP.
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50 nM of the indicated AGUAA-FAM, pAGUAA-FAM, ppAGUAA-FAM, or 10 nM GTP-Bodipy were incubated with increasing concentrations of the indicated protein for 1 hr at 28°C then fluorescence polarization signal was detected. KD and standard deviation values are reported for each. n = 3.
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2015-12-02



