Binding affinities of mutant dengue capping enzyme proteins for different diphosphorylated RNA species and GTP.

50 nM of the indicated AGUAA-FAM, pAGUAA-FAM, ppAGUAA-FAM, or 10 nM GTP-Bodipy were incubated with increasing concentrations of the indicated protein for 1 hr at 28°C then fluorescence polarization signal was detected. KD and standard deviation values are reported for each. n = 3.将50 nM 所示的AGUAA-FAM、pAGUAA-FAM、ppAGUAA-FAM，或10 nM GTP-Bodipy与浓度梯度递增的指定蛋白在28℃条件下孵育1小时，随后检测荧光偏振信号。各组均报告了解离常数（KD）与标准差，实验重复次数n=3。