Sensory nerve niche regulates mesenchymal stem cell homeostasis via FGF/mTOR/autophagy axis [RNA-seq]

The goal of this study is to compare transcriptome profiling (RNA-seq) of proximal end of incisor 1 week after TMX induction between control and Fgfr1 mutant mice Overall design: mRNA profiles of control and Fgfr1 mutant mice were generated by deep sequencing, in triplicate, using NextSeq500 High Output. The quality of RNA samples was determined using an Agilent 2100 Bioanalyzer and all samples for sequencing had RNA integrity numbers >9.

本研究旨在对比对照组与Fgfr1突变小鼠在他莫昔芬（TMX）诱导1周后门牙近端的转录组分析（RNA-seq）结果。整体实验设计：采用NextSeq500高输出测序平台，对对照组与Fgfr1突变小鼠的mRNA表达谱进行深度测序，每组设置3次生物学重复。通过安捷伦2100生物分析仪检测RNA样品质量，所有用于测序的样品其RNA完整性评分均大于9。