Gallus gallus gallus Transcriptome or Gene expression

The purpose of this research was to evaluate how a probiotic stimulates immune cells, and to understand what these changes mean for the cell. Cells were stimulated with serum from probiotic-fed animals or control animals, and RNA was isolated and sequenced on an Illumina HiSeq. 4 serum treatments with 3 replicates and 2 time points.本研究旨在评估益生菌对免疫细胞的刺激作用，并阐明此类细胞变化的生物学意义。实验采用益生菌饲喂组与对照组动物的血清分别刺激免疫细胞，随后提取RNA并在Illumina HiSeq测序平台上进行测序。本次实验共设置4组血清处理条件，每组设置3次生物学重复，并包含2个采样时间点。