RNA deep sequencing analysis of human brain microvascular endothelial cells (ECs) treated with glioma-conditioned medium (glioma-CM)

To determine the effects of glioma-conditioned medium on global gene expression in ECs. Overall design: Three lines of human microvascular ECs were treated with or without glioma-CM, followed by RNA extraction and RNA-seq.

本研究旨在探究胶质瘤条件培养基（glioma-conditioned medium）对内皮细胞（Endothelial Cells, ECs）全局基因表达的影响。实验整体设计：选取三株人微血管内皮细胞，分为两组分别经胶质瘤条件培养基（glioma-CM）处理与未处理（空白对照），随后提取RNA并进行RNA测序（RNA-seq）。