Additional file 1: of The E3 ubiquitin ligase RNF40 suppresses apoptosis in colorectal cancer cells

Figure S1. Reduction of RNF40 and H2Bub1 in siRNF40 CRC cell lines. (A-B). The siRNA-mediated knockdown of RNF40 was verified at the mRNA level using qRT-PCR (A) and on protein level using western blot (B) in three independent experiments 72 h after transfection. Mean ± SEM, Students t test. (C) The knockdown of RNF40 resulted in decreased H2Bub1 levels 72 h after siRNA transfection. (ZIP 7432 kb)图S1：siRNF40处理的结直肠癌（CRC）细胞系中RNF40与H2Bub1的表达下调。(A)-(B) 转染后72小时，通过3次独立实验，分别采用实时定量逆转录PCR（quantitative real-time reverse transcription PCR, qRT-PCR）(A) 检测mRNA水平、蛋白质印迹（western blot）(B) 检测蛋白水平，以验证siRNA介导的RNF40敲低效果。实验数据以平均值±标准误（SEM）表示，采用Student t检验进行统计学分析。(C) 在siRNA转染72小时后，RNF40的敲低会导致H2Bub1的表达水平降低。（ZIP压缩文件，大小7432 kb）