Effect of mutant GNAS expression in the doxycycline-inducible Kras;Gnas mouse model of IPMN using scRNA-seq

We investigated the effect of GNAS(R201C) expression in the Kras;Gnas model of pancreatic intraductal papillary mucinous neoplasms where transgenic mutant GNAS is doxycycline inducible (LGKC; p48(Cre), Kras(LSL-G12D), Rosa26(LSL-rtTA)), Tg(TetO-GNAS(R201C)) using scRNA-seq of dissociated pancreatic tissues. Four Kras;Gnas mice were fed with doxycycline or normal lab feed for 10 weeks starting at 8 weeks of age (2 mice per group). Pancreata were dissociated and subjected to scRNA-seq.

我们采用解离胰腺组织的单细胞RNA测序(single-cell RNA sequencing, scRNA-seq)技术，探究了胰腺导管内乳头状黏液瘤的Kras;Gnas模型中GNAS(R201C)的表达效应，该模型的转基因突变GNAS可通过多西环素诱导（LGKC；p48(Cre)、Kras(LSL-G12D)、Rosa26(LSL-rtTA)）且携带Tg(TetO-GNAS(R201C))。我们将4只Kras;Gnas小鼠从8周龄开始分为两组（每组2只），分别喂食多西环素饲料与普通实验饲料，持续干预10周；随后分离胰腺组织并进行单细胞RNA测序。