Table_4_The Expression Profiles of mRNAs and lncRNAs in Buffalo Muscle Stem Cells Driving Myogenic Differentiation.xls

Buffalo breeding has become an important branch of the beef cattle industry. Hence, it is of great significance to study buffalo meat production and meat quality. However, the expression profiles of mRNA and long non-coding RNAs (lncRNA) molecules in muscle stem cells (MuSCs) development in buffalo have not been explored fully. We, therefore, performed mRNA and lncRNA expression profiling analysis during the proliferation and differentiation phases of MuSCs in buffalo. The results showed that there were 4,820 differentially expressed genes as well as 12,227 mRNAs and 1,352 lncRNAs. These genes were shown to be enriched in essential biological processes such as cell cycle, p53 signaling pathway, RNA transport and calcium signaling pathway. We also identified a number of functionally important genes, such as MCMC4, SERDINE1, ISLR, LOC102394806, and LOC102403551, and found that interference with MYLPF expression significantly inhibited the differentiation of MuSCs. In conclusion, our research revealed the characteristics of mRNA and lncRNA expression during the differentiation of buffalo MuSCs. This study can be used as an important reference for the study of RNA regulation during muscle development in buffalo.

水牛育种现已成为肉牛产业的重要分支，因此开展水牛肉生产与肉品质相关研究具有重要意义。然而，目前针对水牛肌肉干细胞（muscle stem cells, MuSCs）发育过程中mRNA与长链非编码RNA（long non-coding RNAs, lncRNA）的表达谱研究尚未得到充分探索。为此，本研究对水牛MuSCs增殖与分化阶段的mRNA及lncRNA表达谱进行了分析。研究结果显示，共鉴定得到4820个差异表达基因，以及12227条mRNA和1352条lncRNA。富集分析表明，这些基因显著富集于细胞周期、p53信号通路、RNA转运及钙信号通路等关键生物学过程。此外，本研究还鉴定出多个功能重要的基因，包括MCMC4、SERDINE1、ISLR、LOC102394806及LOC102403551，并发现干扰MYLPF的表达可显著抑制MuSCs的分化。综上，本研究揭示了水牛MuSCs分化过程中mRNA与lncRNA的表达特征，可为水牛肌肉发育过程中的RNA调控研究提供重要参考依据。