HEA acts against P. xylostella via adenosine receptors

The diamondback moth, Plutella xylostella, is one of the most important pests of cruciferous crops. We have earlier shown that N6-(2-hydroxyethyl)-adenosine (HEA) exhibits insecticidal activity against P. xylostella. In the present study we investigated the possible mechanism of insecticidal action of HEA on P. xylostella. HEA is a derivative of adenosine, therefore, we speculated whether it acts via P. xylostella adenosine receptor (PxAdoR). We used RNAi approach to silence PxAdoR gene and used antagonist of denosine receptor (AdoR) to study the insecticidal effect of HEA. We cloned the whole sequence of PxAdoR gene.小菜蛾（Plutella xylostella）是十字花科作物的主要害虫之一。本团队前期研究发现，N6-(2-羟乙基)-腺苷（HEA）对小菜蛾具有杀虫活性。本研究旨在探究HEA对小菜蛾的杀虫作用潜在机制。鉴于HEA为腺苷的衍生物，我们推测其可能通过小菜蛾腺苷受体（PxAdoR）发挥作用。我们采用RNA干扰（RNAi）技术沉默PxAdoR基因，并使用腺苷受体（AdoR）拮抗剂来研究HEA的杀虫效果。我们还克隆了PxAdoR基因的全长序列。