Gene Regulation of Intestinal Porcine Epithelial Cells IPEC‑J2 is Dependent on the Site of Deoxynivalenol Toxicological Action. Sus scrofa

Here we analysed different mechanisms of apical and basolateral deoxynivalenol (DON) toxicity reflected in the gene expression. We used the jejunum derived, polarized intestinal porcine epithelial cell line IPEC‑J2 as an in vitro cell culture model. Luminal and blood stream DON intoxication was mimicked by a DON application from the apical or basolateral compartment of ThinCert® membrane inserts for 72 h. Overall design: We compared the genome wide gene expression of untreated and DON-treated IPEC-J2 cells by the GeneChip® Porcine Genome Array of Affymetrix.

本研究分析了由基因表达反映出的顶侧与基底侧脱氧雪腐镰刀菌烯醇（deoxynivalenol，DON）毒性的不同作用机制。本研究采用源自空肠的极化猪肠道上皮细胞系IPEC-J2作为体外细胞培养模型。通过向ThinCert®膜插入件的顶侧或基底侧隔间添加脱氧雪腐镰刀菌烯醇，模拟肠腔与血流中的脱氧雪腐镰刀菌烯醇染毒过程，染毒时长为72小时。实验整体设计：采用Affymetrix的GeneChip®猪基因组芯片，对比未处理与经脱氧雪腐镰刀菌烯醇处理的IPEC-J2细胞的全基因组基因表达水平。