WT vs KO hdiR comparison

Transcriptional profiling of S. thermophilus LMD-9 wild-type compared to the isogenic mutant strain CB0082 (deletion of hdiR, STER_0918) for the identification of the HdiR regulon. Cells were grown in CDM medium supplemented with lactose 1% (CDML) and sampled at OD600 = 0.4 for mRNA extraction wild-type vs knockout, 2 WT biological replicates (one used as technical replicate for a dye swap), 2 KO biological replicates (one used as technical replicate for a dye swap)

本研究针对嗜热链球菌（Streptococcus thermophilus）LMD-9野生型与其同基因敲除突变株CB0082（hdiR基因缺失，STER_0918）开展转录谱分析，以鉴定HdiR调节子（HdiR regulon）。实验菌株于添加1%乳糖的化学限定培养基（CDM）中培养，该培养基简称CDML，在光密度OD600=0.4时取样以提取mRNA，用于野生型与敲除株的转录组比对。其中野生型设置2次生物学重复（其中1次作为染料互换的技术重复），敲除株同样设置2次生物学重复（其中1次作为染料互换的技术重复）。