16S rRNA gene amplicon sequencing of gut microbiota in a spontaneous EAE mouse model. mouse gut metagenome

We studied the effect of high salt diet on the gut microbiota of opticospinal encephalomyelitis mice (double-transgenic C57BL/6 mice). Small intestinal (n=8), large intestinal (n=8) and cecal (n=16) microbiota were profiled using 16S rRNA gene sequencing targetting V3-V4 hyper-variable regions.

本研究探讨了高盐饮食对双转基因C57BL/6小鼠视神经脊髓炎（opticospinal encephalomyelitis）模型肠道菌群的影响。实验采用靶向V3-V4高变区（V3-V4 hyper-variable regions）的16S rRNA基因测序（16S rRNA gene sequencing），对小肠（n=8）、大肠（n=8）及盲肠（n=16）的肠道菌群进行了群落谱分析。