mRNA expression data of benzene exposed mice and IGF2BP1 overexpressed mice

Benzene induced bone marrow damage, and IGF2BP1 was reduced in mice. IGF2BP1 is a RNA binding protein, and can regulate the RNA expression. Therefore, we constructed IGF2BP1 overexpression mice to explore the role of IGF2BP1 We used microarrays to detail the global programme of gene expression after benzene exposure and identified distinct classes of IGF2BP1-regulated genes during this process. Mice were randomly divided into 4 groups (10 mice per group): (1) control group (AAV‐null), (2) control-AAV‐IGF2BP1 group, (3) benzene group (benzene + AAV‐null), and (4) benzene-AAV‐IGF2BP1 group. AAV‐null (2.5×1011viruses in 200 μL normal saline), or AAV‐ IGF2BP1 (2.5 ×1011 viruses in 200 μL normal saline) were injected through the tail vein using sterile disposable syringes. Two-week post injection, mice were exposed to 0 and 50 ppm benzene for 6 h/ day, 6 days/week and 9 weeks. The femur and tibia of mice were collected. The bone was cut off at both ends and bone marrow was flushed out with sterile phosphate buffered saline (PBS) using a syringe. Bone marrow cells were obtained by filtrating through a 70 μm cell strainer (SORFA, Beijing, China). After centrifugation at 1500 rpm for 5 min, cells were resuspended for counting. The total RNA was isolated from 1 × 106 bone marrow cells using TRIzol reagent (Invitrogen, CA, USA) for Affymetrix microarray profiling.

苯暴露可诱导小鼠骨髓损伤，且小鼠体内胰岛素样生长因子2 mRNA结合蛋白1（IGF2BP1）表达水平降低。IGF2BP1作为一种RNA结合蛋白，可调控RNA的表达。为此，我们构建了IGF2BP1过表达小鼠模型，以探究IGF2BP1的生物学功能。我们通过微阵列技术全面解析了苯暴露后的小鼠全局基因表达谱，并鉴定出该过程中受IGF2BP1调控的不同类别基因。将小鼠随机分为4组（每组10只）：(1) 空载体对照组（AAV-null），(2) AAV-IGF2BP1对照组，(3) 苯暴露组（苯+AAV-null），以及(4) 苯暴露+AAV-IGF2BP1组。采用无菌一次性注射器经尾静脉注射AAV-null（2.5×10^11病毒颗粒溶于200 μL生理盐水）或AAV-IGF2BP1（2.5×10^11病毒颗粒溶于200 μL生理盐水）。注射2周后，将小鼠分别暴露于0 ppm与50 ppm的苯环境中，每日暴露6小时，每周6天，持续9周。收集小鼠的股骨与胫骨，切断两端后以无菌磷酸盐缓冲液（PBS）通过注射器冲洗获取骨髓。经70 μm细胞筛（SORFA，中国北京）过滤分离骨髓细胞，1500 rpm离心5分钟后重悬细胞并进行计数。从1×10^6个骨髓细胞中提取总RNA，使用TRIzol试剂（Invitrogen，美国加利福尼亚州）完成Affymetrix微阵列基因表达谱分析。