Binding of 146S and 12S particles onto the ELISA plates.

Excess amount of antigen was coated ON using either PBS or Carbonate/bicarbonate buffer. Plates were washed after the ON incubation at 4°C and total coated proteins were quantified using a commercial kit.过量抗原采用磷酸盐缓冲液（PBS）或碳酸盐/碳酸氢盐缓冲液进行过夜包被。4℃过夜孵育后，对微孔板进行洗涤，并使用商用试剂盒对总包被蛋白进行定量检测。