Effect of 30Kc19α-Lin28A protein on gene expression in osteogenesis of urine-derived stem cells. Effect of 30Kc19α-Lin28A protein on gene expression in osteogenesis of urine-derived stem cells

To elucidate the function of 30Kc19α-Lin28A protein in osteogenic differentiation of urine-derived stem cells, we established urine-derived stem cell lines differentiated with or without protein treatment. We then performed gene expression profiling analysis using data obtained from RNA-seq of osteogenic differentiated urine-derived stem cells with or without 30Kc19α-Lin28A protein treatment, undifferentiated urine-derived stem cells, and human osteoblasts Overall design: Comparative gene expression profiling analysis of RNA-seq data for urine-derived stem cells differentiated into osteoblasts with or without treatment with 30Kc19α-Lin28A protein.

为阐明30Kc19α-Lin28A蛋白在尿源干细胞（urine-derived stem cells）成骨分化（osteogenic differentiation）中的功能，我们构建了尿源干细胞系，并将其分为两组：一组在成骨分化过程中辅以30Kc19α-Lin28A蛋白处理，另一组不施加该蛋白处理。随后，我们针对以下样本的RNA测序（RNA-seq）数据开展基因表达谱分析：经30Kc19α-Lin28A蛋白处理的成骨分化尿源干细胞、未施加蛋白处理的成骨分化尿源干细胞、未分化的尿源干细胞，以及人成骨细胞。整体实验设计：对经或未经30Kc19α-Lin28A蛋白处理、并诱导分化为成骨细胞的尿源干细胞的RNA测序（RNA-seq）数据进行比较基因表达谱分析。