WSMV and/or TriMV infection affected biogenesis of vsiRNAs in wheat cv. Arapahoe and Mace at 27°C and 18°C.

*Small RNA reads normalized using Arapahoe at 18°C as a standard (see Table S2).
aAr: Wheat cv. Arapahoe; Mc: Wheat cv. Mace; SI: single infection with either WSMV or TriMV; DI: double infection with WSMV and TriMV.
bsmall RNAs that are not aligned with virus genomes are considered as endogenous small RNAs.
cvirus specific siRNAs to WSMV and TriMV.
dPercent vsiRNAs accumulation compared to endogenous RNAs.
WSMV and/or TriMV infection affected biogenesis of vsiRNAs in wheat cv. Arapahoe and Mace at 27°C and 18°C.

*以18℃下的阿帕霍小麦（Arapahoe）为参照样本完成小RNA（small RNA）读长的标准化处理（详见附表S2）。
a 供试材料与处理分组说明：aAr指代小麦品种阿帕霍（cv. Arapahoe），Mc指代小麦品种梅斯（cv. Mace）；SI为单一侵染处理，即仅接种WSMV或TriMV；DI为复合侵染处理，即同时接种WSMV与TriMV。
b 未比对至病毒基因组的小RNA被定义为内源性小RNA。
c 靶向WSMV与TriMV的病毒特异性小干扰RNA（siRNA）。
d 病毒来源小干扰RNA（vsiRNA）相对于内源性RNA的积累百分比。
27℃与18℃条件下，WSMV和/或TriMV侵染均可影响小麦品种阿帕霍与梅斯体内vsiRNA的生物发生过程。