Mammary carcinomas in WAP-SV40 transgenic mice [gene expression]. Mus musculus

Transgenic expression in mice of two synergistically acting SV40 early region encoded proteins, large (LT) and small (sT) tumor antigens, in the mammary epithelium recapitulates loss of p53 and Rb function and deregulation of PP2A-controlled mitogenic pathways in human breast cancer. In primiparous mice, WAP-promoter driven expression of SV40 proteins induces well and poorly differentiated mammary adenocarcinomas. We performed a correlative aCGH and gene expression analysis of 25 monofocal tumors, representing four histopathological grades, to explore the molecular traits of SV40-induced mammary tumors and to emphasize the relevance of this tumor model for human breast tumorigenesis. Overall design: Gene expression analysis of 25 mammary carcinoma samples of two different WAP-SV40 mouse lines, T1 and NP8. Three involuted mammary gland tissues were included in the study as reference samples.

在小鼠乳腺上皮中转基因表达两种协同作用的SV40早期区编码蛋白——大T抗原（LT）与小T抗原（sT）——可重现人类乳腺癌中p53与Rb功能丧失以及蛋白磷酸酶2A（PP2A）调控的有丝分裂原通路失调的病理表型。
对于初产小鼠，受WAP启动子驱动的SV40蛋白表达可诱导高分化与低分化两种亚型的乳腺腺癌。
本研究针对25例单灶性乳腺肿瘤开展了关联性阵列比较基因组杂交（aCGH）与基因表达分析，所纳入的肿瘤涵盖4种组织病理学分级，旨在探究SV40诱导型乳腺肿瘤的分子特征，并凸显该肿瘤模型对人类乳腺肿瘤发生研究的相关性价值。
整体实验设计：对来自T1与NP8两个不同WAP-SV40小鼠品系的25例乳腺腺癌样本进行基因表达分析，本研究同时纳入3例退化期乳腺组织作为对照样本。