Effect of knockdown or overexpression of CYP1B1 on gene expression during proliferation process of colorectal cancer cells (RKO and SW480).. Effect of knockdown or overexpression of CYP1B1 on gene expression during proliferation process of colorectal cancer cells (RKO and SW480).

To investigate the function of CYP1B1 in the regulation of proliferation process, we established SW480 cells in which target gene has been knocked down by shRNA and established RKO cells in which target gene has been upregulated by overexpressed plasmid. Overall design: We performed gene expression profiling analysis using data obtained from RNA-seq of 4 different cells (RKO-Control vs RKO-CYP1B1, SW480-shNT vs SW480-shCYP1B1).

为探究细胞色素P450 1B1（CYP1B1）在细胞增殖调控过程中的功能，我们构建了经短发夹RNA（shRNA）敲低CYP1B1的SW480细胞系，以及通过过表达质粒上调CYP1B1的RKO细胞系。整体实验设计：我们针对4组细胞的RNA测序（RNA-seq）数据开展基因表达谱分析，分析分组为RKO对照组（RKO-Control）与RKO-CYP1B1过表达组（RKO-CYP1B1）、SW480阴性对照短发夹RNA组（SW480-shNT）与SW480-CYP1B1敲低组（SW480-shCYP1B1）。