Reconstruction of hippocampus CA1 stratum oriens, oriens-lacunosum moleculare cell

title : Reconstruction of hippocampus CA1 cell morphologies

specimen : Rattus norvegicus

sex : male

weight : 100-200 g

This neuron was recorded and filled with biocytin in a 450-500 µm thick coronal slice of adult rat hippocampus, using sharp electrodes (for methods see PMID: 11807843).  The fixed slice was resectioned at 50-60 µm and the cell reconstructed with Neurolucida using a 100x oil immersion objective.

标题：海马体CA1神经元形态重构
标本：褐家鼠（Rattus norvegicus）
性别：雄性
体重：100-200克
本神经元使用锐化电极，在成年大鼠海马体450-500微米厚的冠状脑片中完成电生理记录，并向胞内填充生物胞素，具体实验方法详见PMID: 11807843。固定后的脑片被再次切片至50-60微米厚度，随后使用100倍油浸物镜，通过Neurolucida软件完成该神经元的形态重构。