DDX20 is required for cell cycle reentry of prospermatogonia and establishment of spermatogonial stem cell pool during testicular development in mice

DDX20 is required to promote spermatogonial stem cell pool formation by regulating mRNA translation during postnatal prospermatogonia differentiation in mice. We used THY1-positive cells isolated from Ddx20 knockout mice and littermate controls for low-input Ribo-seq.

在小鼠中，DDX20是通过调控出生后前精原细胞（prospermatogonia）分化过程中的mRNA翻译（mRNA translation）以促进精原干细胞池（spermatogonial stem cell pool）形成所必需的因子。我们使用从Ddx20基因敲除小鼠及其同窝对照小鼠中分离得到的THY1阳性细胞，开展低起始量核糖体测序（Ribo-seq）实验。