Triticum aestivum cv. Chinese Spring Genome sequencing and assembly. Triticum aestivum cultivar:Chinese Spring

Assembling complete genomes for large and intricate plant species remains a formidable task, and the hexaploid wheat genome poses a particularly challenging hurdle among essential food crops. To overcome this challenge, we employed a complementary approach that integrates PacBio HiFi and ONT ultra-long (UL) read sequencing, along with crucial datasets such as chromosome conformation capture (Hi-C), Illumina, and Bionano. Our achievement presents a comprehensive telomere-to-telomere (T2T) wheat genome with no gaps and accurately includes all 21 centromeres and 42 telomeres.

组装大型复杂植物物种的完整基因组仍是一项极具挑战性的任务，六倍体小麦基因组在关键粮食作物中更是一道尤为棘手的障碍。为攻克这一难题，我们采用了整合PacBio 高保真测序（PacBio HiFi）与牛津纳米孔超长读长（ONT ultra-long, UL）测序的互补策略，并辅以染色体构象捕获（Hi-C）、Illumina测序及Bionano图谱等关键数据集。本研究成功获得了无间隙的端粒到端粒（T2T）小麦基因组，精准囊括全部21个着丝粒与42个端粒。