Immune-stimulated Peyer's patch. Sus scrofa

RNA from all normal tissues were pooled in equal amounts and used to create the normal tissue library. RNA from all immune stimulated tissues (Salmonella infection, CT+LPS, and PBC, with and without cycloheximide) were pooled in equal amounts and used to create the immune stimulated tissue library. The subtracted library was created by combining the normal and immune stimulated libraries and by subtracting porcine fibroblast RNA. Keywords: other

将所有正常组织的核糖核酸（RNA）按等量混匀，用于构建正常组织文库。将所有免疫刺激组织样本（包括沙门氏菌感染模型、CT+LPS处理组、PBC模型，以及添加与未添加环己酰亚胺的组别）的RNA按等量混匀，用于构建免疫刺激组织文库。通过混合正常组织文库与免疫刺激组织文库，并去除猪成纤维细胞RNA，构建得到消减文库。关键词：其他