Rsd timecourse

The gene expression profile of MC4100 delta rsd with pACYC Rsd and pACYC delta BS plasmids has been analysed using whole genome oligonucleotide microarrays. Control RNA was isolated from 50ml LB cultures of MC4100 delta rsd strain with pACYC delta BS. Experimental RNA was isolated from 50ml LB cultures of MC4100 delta rsd with pACYC Rsd, for each growth condition (OD600 = 0.4: log phase, 0.9: late log phase, 1.1: transition phase, ST(OD600 = >2.0): stationary phase). The experiments performed replicate. Keywords: timecourse (overproduction of Rsd) timecourse = OD600:0.4,0.9,1.1,ST(>2.0) replicate = 2

本研究采用全基因组寡核苷酸微阵列（whole genome oligonucleotide microarray）技术，对携带pACYC Rsd质粒与pACYC ΔBS质粒的MC4100 Δrsd菌株的基因表达谱进行了分析。对照RNA提取自携带pACYC ΔBS质粒的MC4100 Δrsd菌株的50 mL LB培养基培养物；实验组RNA则提取自携带pACYC Rsd质粒的MC4100 Δrsd菌株的50 mL LB培养基培养物，涵盖四种生长条件：600nm光密度（OD600）为0.4（对数生长期）、0.9（对数晚期）、1.1（过渡生长期）以及ST（OD600>2.0，稳定生长期）。本实验设置了生物学重复。关键词：时间进程（Rsd过表达），其中时间进程对应OD600值为0.4、0.9、1.1及ST（>2.0），重复次数为2。