Expression of transport proteins in the rete mirabile of European silver and yellow eel

We investigated transcriptional and translational changes in rete mirabile tissue of European yellow and silver eels. We therefore analyzed the transcriptome as well as the proteome of rete capillaries of specimens of both developmental stages. The results clearly show the presence of a large number of membrane transport proteins in the transcriptome as well as in the proteome. ATP required to energize ion and metabolite transport can be generated in the aerobic metabolism. A high ROS defense capacity is established in rete endothelial cells, probably connected to the hyperbaric oxygen partial pressures, generated in the rete by back-diffusion of oxygen. Using Illumina Sequencing to obtain RNA-Seq data and statistical Deseq analysis, the transcriptomes of European eel rete mirabile tissues of animals of different developmental stages were compared. Using LC-MS/MS and statistical analysis, the proteomes of European eel rete mirabile tissues of animals of different developmental stages were compared.

本研究针对欧洲黄鳗与银鳗的迷网（rete mirabile）组织中的转录及翻译水平变化展开探究。为此，我们对两个发育阶段标本的迷网毛细血管的转录组（transcriptome）与蛋白质组（proteome）开展了分析。分析结果清晰表明，转录组与蛋白质组中均存在大量膜转运蛋白（membrane transport proteins）。为离子与代谢物转运供能所需的三磷酸腺苷（ATP）可通过有氧代谢生成。迷网内皮细胞具备高水平的活性氧（reactive oxygen species, ROS）防御能力，该能力大概率与迷网内因氧反向扩散形成的高压氧分压密切相关。本研究采用Illumina测序（Illumina Sequencing）获取RNA测序（RNA-Seq）数据并结合DESeq统计分析，对不同发育阶段欧洲鳗鲡迷网组织的转录组进行了比较分析。本研究采用液相色谱-串联质谱（liquid chromatography-tandem mass spectrometry, LC-MS/MS）并结合统计分析，对不同发育阶段欧洲鳗鲡迷网组织的蛋白质组进行了比较分析。