The Development of a Next Generation Sequencing Panel Targeting Cannabinoid Synthase Genes to Distinguish Between Marijuana and Hemp

A custom Next Generation Sequencing (NGS) panel was developed targeting all synthase genes (THCAS, CBDAS, and CBCAS) and pseudogenes across diverse C. sativa samples, spanning reference hemp and marijuana materials, commercial hemp derivatives, and seized marijuana extracts. This bioproject contains raw sequencing reads from the MiSeq FGx.

本研究开发了一款定制化下一代测序（Next Generation Sequencing, NGS）面板，靶向覆盖多种大麻（Cannabis sativa）样本中的全部合酶基因（THCAS、CBDAS及CBCAS）及其假基因；所纳入的样本涵盖参考级工业大麻与大麻材料、商用工业大麻衍生物以及缉获的大麻提取物。本生物项目包含MiSeq FGx测序平台产出的原始测序读段。