CIL:38670, Rattus rattus, glandular epithelial cell, milk secreting cell, mammary alveolar cell. In Cell Image Library

Immunoelectron microscopy of mammary gland tissue from lactating rats investigating potential structural interactions between mature lipid droplets and elements of the trans-Golgi network, identified by TGN38 (a transmembrane trans-Golgi protein; larger gold particles) and cation-dependent mannose-6-phosphate receptor (an integral membrane protein that localizes to the trans-Golgi reticulum, endosomes, and the plasma membrane; smaller gold particles) staining. Note that trans‚ÄêGolgi cisternae in lactating mammary cells contain large amounts of casein and other milk proteins and are grossly expanded due to the osmolarity of the secreted fluid.

本数据集针对泌乳大鼠的乳腺组织开展免疫电子显微镜成像，旨在探究成熟脂滴与反式高尔基体网络（trans-Golgi network, TGN）各组分间潜在的结构相互作用。实验通过两种免疫金染色标记物实现相关组分的识别：其一为TGN38（一种跨膜反式高尔基体蛋白，以较大金颗粒作为标记信号）；其二为阳离子依赖型甘露糖-6-磷酸受体（一种定位于反式高尔基体网状结构、内体与细胞质膜的整合膜蛋白，以较小金颗粒作为标记信号）。需注意，泌乳乳腺细胞内的反式高尔基体潴泡富含酪蛋白与其他乳源蛋白，且因分泌液的渗透压而发生显著扩张。