SAIMOS - Biological and Flow Cytometry data collected from CTD stations in South Australia, in December 2008

Flow cytometry data was collected in December 2008, in waters off South Australia. The general purpose of the study is to be able to establish background knowledge on the ecosystem on the continental shelf of South Australia and the impact of upwelling/saline outflow events on microbial communities to ultimately develop a biogeochemical model of the region. Sampling was carried out during cruises conducted on board the RV Ngerin as part of the Southern Australian Integrated Marine System (SAIMOS). During each cruise, the physical, chemical and biological properties of the chlorophyll fluorescence maximum (FM) layer were investigated. Flow cytometry data has been collected for picophytoplankton (not bacteria and viruses this cruise).Six main stations have been sampled over the course of the study, five are located on the 100 m isobath, i.e. RS (35.508S, 136.278E), B2 (35.418S, 136.148E), B3 (35.258S, 136.048E), B4 (35.168S, 135.418E) and B5 (35.008S, 135.198E), and one from an offshore station (B1; 36.188S, 136.178E) located southwest of Kangaroo Island. Note that combining the distances between stations (14–25 nautical miles), the average component of the current velocity at middepth along the shelf (0.01 m s21) and the average speed of the vessel (i.e. 9 knots) indicate that different water masses were sampled at each station. Additional samples have on occasion been collected from the National Reference Station (NRS) at Kangaroo Island (35.832S, 136.447E) and the SA Spencer Gulf Mouth Mooring (SAM8SG, 35.25S, 136.690E), where the saline outflow occurs.

本数据集的流式细胞术（Flow Cytometry）数据采集于2008年12月，采集区域为澳大利亚南部外海水域。本研究的核心目标为：建立澳大利亚南部大陆架海域生态系统的背景知识体系，阐明上升流/咸水流出事件对微生物群落的影响，最终构建该区域的生物地球化学模型。采样工作依托澳大利亚南部综合海洋系统（Southern Australian Integrated Marine System, SAIMOS）项目，在RV Ngerin号科考船上开展的多个航次中完成。每个航次中，研究人员均对叶绿素荧光最大值层（Chlorophyll Fluorescence Maximum, FM）的物理、化学及生物学特征展开调查。本次航次采集的流式细胞术数据仅针对微微型浮游植物（Picophytoplankton），未包含细菌与病毒样本。本研究共布设6个主要采样站位：其中5个站位位于100米等深线处，分别为RS（35.508°S，136.278°E）、B2（35.418°S，136.148°E）、B3（35.258°S，136.048°E）、B4（35.168°S，135.418°E）及B5（35.008°S，135.198°E）；剩余1个为离岸站位B1（36.188°S，136.178°E），位于袋鼠岛（Kangaroo Island）西南海域。需注意的是，结合站位间距（14~25海里）、陆架中层海流的平均流速分量（0.01 m·s⁻¹）以及科考船的平均航速（9节），可推断每个站位所采集的水团均不相同。此外，研究人员偶尔会从袋鼠岛国家参考站（National Reference Station, NRS，35.832°S，136.447°E）以及发生咸水流出事件的南澳大利亚斯潘塞湾口锚站（SA Spencer Gulf Mouth Mooring, SAM8SG，35.25°S，136.690°E）采集补充样本。