Spatiotemporal resolution of germinal center Tfh cell differentiation and divergence from central memory CD4+ T cell fate. Spatiotemporal resolution of germinal center Tfh cell differentiation and divergence from central memory CD4+ T cell fate

Transcriptome analysis of CD4+ T cells responding to influenza infection. Overall design: Purified naïve OT-II T cells were transferred into CD45.1+ SMARTA recipient mice followed by intranasal infection with PR8-OVA. The donor OT-II cells from the medLN of the recipient mice were analyzed by RNA-seq at day 21 p.i.

本数据集聚焦于应答流感感染的CD4+ T细胞的转录组分析。实验设计概述：将纯化的初始OT-II T细胞移植至CD45.1阳性SMARTA受体小鼠体内，随后经鼻内感染PR8-OVA病毒；于感染后（post infection，p.i.）第21天，对受体小鼠纵隔淋巴结（medLN）中的供体OT-II细胞开展RNA测序（RNA-seq）分析。